Fig.6 THBS2 may be associated with CAFs phenotype conversion in the colon cancer microenvironment. 

(A) Monocle₂-mapped cell differentiation trajectory of CAFs colored by each subtypes (top) and pseudo-time (bottom). (B) Expression changes of TSPs within each CAFs subtypes as pseudo-time progresses (Each dot for an individual cell). (C) Identification of five CAFs subtypes enriched by Gene Ontology (GO). (D) The top 50 genes with the most significant expression changes over pseudo-time (q < 0.01) illustrated by the heatmap. (E) Expression changes of key genes in the branch one node over pseudo-time (q < 0.01) using the heatmap. (F) Co-expression patterns of TSPs with branch one node genes (*, p < 0.05; **, p < 0.01; ***, p < 0.001). (G) The top 10 Differentially Expressed Genes (DEGs) among the five CAFs subtypes in the GSE166555 cohort (log₂FC > 1.8 and p < 0.01).

Fig. S7: Spatial localization of gene markers and the direction of the CAFs cell trajectory.

(A) Spatial distribution of TSPs (THBS2,THBS4, and COMP) and CAFs markers in colon cancer tissue, with red indicating higher gene expression levels. (B) CytoTRACE scores for five CAFs subtypes. (C) Evaluation of cell differentiation using Slingshot. (D) Distribution of cell groups corresponding to each state in the cohort(GSE166555, n=12).